C6-NBD Ceramide
Fluorescent Golgi probe / C6-NBD Ceramide is a selective stain for the Golgi apparatus.1 Readily taken up by MDCK cells and converted into C6-NBD-sphingomyelin and C6-NBD-glucosyl-ceramide.2 A useful substrate for assay of ceramide kinase.3 May be used to monitor the biosynthesis and degradation of sphinogmyelin in cellular systems.4 Ex: 455 nm and Em: 533 nm.4
Biochemicals & reagents
94885-02-6
N-(NBD-aminohexanoyl) D-erythro-sphingosine; C6-NBD-Cer
1 Chazotte (2012), Labeling Golgi with Fluorescent Ceramides; Cold Spring Harb. Protoc. (8):pdb.prot070599 2 van Meer et al. (1987), Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells; J. Cell Biol. 105 1623 3 Don and Rosen (2008), A fluorescent plate reader assay for ceramide kinase; Anal. Biochem. 375 265 4 Nikolova-Karakashian (2021), Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics; Methods Mol. Biol. 2187 113
-20°C
TARGET: Substrate / Probe -- PATHWAY: Fatty acid metabolism