Mitochondrial membrane potential probe / Dual emission, potential-sensitive, carbocyanine probe which can be used to measure mitochondrial membrane potential. When excited at 490 nm the monomer exhibits an emission maximum at 527 nm and J-aggregates at 590 nm.1,2 JC-1 changes color reversibly from green to red with increasing mitochondrial membrane potential.3 May be used for isolation of live cells expressing high multidrug resistance transport activity.4 A widely used probe for determination of mitchondrial membrane potential.5
Biochemicals & reagents
3520-43-2
1) Reers et al. (1991), J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential; Biochemistry, 30 4480 2) Smiley et al. (1991), Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1; Proc. Natl. Acad. Sci. USA, 88 3671 3) Lisa et al. (1995), Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition; J. Physiol., 486 1 4) Wolosin et al. (2017), Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry; PLoS One, 12(4) e0174905 5) Wang et al. (2017), A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway; J. Transl. Med., 15 33
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TARGET: Substrate / Probe -- PATHWAY: Mitochondrial function