Coelenterazine-h
Cell viability probe / Renilla luciferase catalyzes coelentarazine oxidation by oxygen to produce light. Coelentarazine-h produces 10-fold higher light output than native coelentarazine. Coelentarazine-h also undergoes autoluminescence which is enhanced by superoxide anion and peroxynitrite in cells. May be used in gene reporter assays, live cell assays and biochemical assays (ELISA, BRET etc).
Biochemicals & reagents
50909-86-9
2-Deoxycoelenterazine
1) Shimomura et al. (1989), Semi-synthetic aequorins with improved sensitivity to Ca2+ ions; Biochem. J., 261 913 2) Nakajima-Shimada et al. (1991), Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system; Proc. Natl. Acad. Sci. USA, 88 6878 3) Inouye et al. (1992), Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin; Anal. Biochem., 201 114
-20°C
TARGET: Substrate / Probe -- RESEARCH AREA: Cell death; Oxidative stress