Cellular proliferation probe / A fluorophore which can diffuse into cells and covalently react with intracellular proteins via its activated carboxy group and thus be retained in the cell for long periods of time.1 CFSE fluorescence can be used to analyze cell proliferation2, track cell migration3 and in applications such as flow cytometry4. Excitation/emission max: 491 and 518 nm.
Biochemicals & reagents
92557-80-7
5-Carboxyfluorescein NHS ester; 5-FAM-SE
1 Breeuwer et al. (1996), A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester; Appl. Environ. Microbiol., 62 178 2 Lyons et al. (2000) Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution; J. Immunol. Methods, 243 147 3 Parrish et al. (2009) Use of the intracellular fluorescent dye CFSE to Monitor lymphocyte migration and proliferation; Curr. Protoc. Immunol., Suppl. 84 4.9.1 4 Kandg et al. (2023) Cytotoxicity of Human Hepatic Intrasinusoidal Gamma/Delta T Cells Depends of Phospho-antigen and NK Receptor Signaling; Anticancer Res., 43 63
-20°C
TARGET: Substrate / Probe -- PATHWAY: Proliferation; Cell migration