RiboGrip® RNase Inhibitor (220 U/µl)
RiboGrip® RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C. RiboGrip® inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. It is primarily used to prevent RNA degradation by contaminating RNases in various assays that use RNA sample materials, such as first strand cDNA synthesis, RT-(q)PCR, RT-LAMP, etc. RiboGrip® also includes a genetic modification - Stability TAG - a proprietary and patented polypeptide stabilization technology [1]. Stability TAG makes RiboGrip® extremely tolerant to higher temperatures and enables room temperature shipping as well as effective use in assays requiring high incubation temperatures. Demonstrated through our in-house testing where RiboGrip® retains full activity after 1 hour incubation at 60 °C.
- Applications:First-strand cDNA synthesis RT-PCR, RT-qPCR, RT-LAMP In vitro transcription and translation RNA isolation and purification RNA sequencing
Molecular Biology
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).
-20°C until Expiry Date; 4°C for 3 months; room temperature for 1 month; Freeze-thaw stability: 30 cycles
Unit definition: One unit is defined as the amount of protein required to inhibit the activity of 5 ng of RNAse A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2',3'‐cyclic monophosphate by RNase A.