FIREPol® DNA Polymerase

Highly processive, thermostable Taq DNA polymerase that provides robust and reproducible results. *Robust amplification in routine applications; *Suitable for templates up to 5 kb; *Reaction set-up and shipment without ice; *TA cloning. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month. Recommended for routine applications (fragment up to 3 kb from genomic DNA). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5).

• Applications:PCR; qPCR; Pre-amplification for Sanger sequencing; Pre-amplification for NGS; Genotyping; Colony PCR; TA cloning