HOT FIREPol® DNA Polymerase

Hot-start DNA Polymerase with a unique 30-day stability at room temperature for your everyday PCR needs. A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up. *Increased specificity and sensitivity; *Reduced primer-dimer formation; *Reaction set-up and shipment without ice. HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb). Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning. The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5).

• Applications:PCR; qPCR; Pre-amplification for Sanger sequencing; Pre-amplification for NGS; Genotyping; Colony PCR; TA cloning