SOLIScript® Fast 1-step RT-qPCR Mix with UNG

Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments. * 4x concentrated one-step RT-qPCR reagent for RNA detection; * Convenient reaction setup with a single vial format; * Amplify up to 5 targets within one reaction; * UNG enzyme prevents carryover contamination and false positive results; * Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST® DNA Polymerase; * Robust performance in inhibited conditions; * RNase inhibitor included in the mix. SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.

• Applications:Detection and quantification of RNA and DNA targets; DNA/LNA hydrolysis probe-based assays; Suitable for singleplex and multiplex reactions (up to 5 plex)