FIREScript® RT cDNA synthesis MIX without primers (100 rxn)
Convenient cDNA synthesis mix with fast and robust FIREScript® Reverse Transcriptase. Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures. * High specificity and yield; * Wide reaction temperature from 37°C to 60°C; * Fast 15 min reaction time; * A convenient 3-vial kit with all reaction components; * RNase inhibitor and water included; * Reaction set-up and shipment without ice. FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
- Applications:First-strand cDNA synthesis; RT-PCR; RT-qPCR
Molecular Biology
-20°C until Expiry Date; 4°C for 6 months; room temperature for 1 month; Freeze-thaw stability: 30 cycles
Unit description: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template in a total reaction volume of 50 μl.