TCEP HCl
Reducing agent / A reagent for selective reduction of disulfides in aqueous solutions1-3 with distinct advantages over DTT which are application specific3. For example, spin labels are more stable with TCEP than DTT, Ni2+ (leached from affinity columns) causes rapid oxidation of DTT with no effect on TCEP and for long term storage of proteins TCEP is more stable than DTT with no metal chelator (EGTA) present but DTT is more stable in the presence of metal chelators.3
Biochemicals & reagents
51805-45-9
1) Fischer et al. (1993), In situ reduction suitable for matrix-assisted laser desorption/ionization and liquid secondary ionization using tris(2-carboxyethyl)phosphine; Rapid Commun. Mass Spectrom., 7 225 2) Kirley et al. (1989), Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analysis; Anal. Biochem., 180 231 3) Getz et al. (1999), A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry; Anal. Biochem., 273 73
-20°C