ML-SA1
TRPML activator / TRPML 1,2 and 3 channel activator.1 Does not activate TRPM2, TRPV2, TRPV3, TRPC6 or TRPA1 channels.2 ML-SA1 robustly activates whole cell TRPML1-4A and whole-endolysosome TRPML1 and is comparable (10 μM) to the effect of the endogenous TRPML agonist, PI(3,5)P2 (1 μM).2 Thus it is a useful chemical tool for studying the functions of TRPMLs. It induces TRPML-mediated Ca2+ release from lysosomes which corrects trafficking defects and reduces cholesterol accumulation in Niemann-Pick type C macrophages.2,3 Reduces intralysosomal Ca2+ level rescuing abnormal lysosomal storage in FIG4-deficient cells.4
Biochemicals & reagents
332382-54-4
1) Grimm et al. (2010), Small molecule activators of TRPML3; Mol. Chem. Biol., 17 135 2) Shen et al. (2012), Lipid storage disorders block lysosomal trafficking by inhibiting a TRP channel and lysosomal calcium release; Nature Commun., 3 731 3) Weiss et al. (2012), Cross-talk between TRPML1 channel, lipids and lysosomal storage diseases; Commun. Integer. Biol., 5 111 4) Zou et al. (2015), Reactivation of lysosomal Ca2+ Efflux Rescues Abnormal Lysosomal Storage in FIG4-Deficient Cells; J. Neurosci.. 35 6801
RT
TARGET: Ion channel; Lysosome -- PATHWAY: Calcium signaling; Cholesterol metabolism -- RESEARCH AREA: Vesicles