Nocodazole
Microtubule inhibitor / Nocodazole is an antimitotic agent that disrupts microtubules by binding to β tubulin and thereby inhibiting microtubule dynamics, disruption of mitotic spindle function, and fragmentation of the Golgi complex.1,2 Arrests cell cycle at G2/M phase. Stimulates the intrinsic GTPase activity of tubulin.3 Nocodazole activates the JNK/SAPK signaling pathway and induces apoptosis in a variety of cell lines.4 Increases Cas9-mediated editing frequencies5 and increased CRISPR-mediated HDR DNA repair6. Cell permeable.
Biochemicals & reagents
31430-18-9
R-17934
1) Jordan et al. (1992), Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis; J. Cell Science, 102 401 2) Storrie et al. (1998), Dynamics of the interphase mammalian Golgi complex as revealed through drugs producing reversible Golgi disassembly; Biochim. Biophys. Acta, 1404 127 3) Mejillano et al. (1996), Studies on the nocodazole-induced GTPase activity of tubulin; Arch. Biochem. Biophys., 336 130 4) Wang et al. (1998), Microtubule-interfering agents activate c-Jun N-terminal kinase/stress-activated protein kinase through both Ras and apoptosis signal-regulating kinase pathways; J. Biol. Chem., 273 4928 5) Lin et al. (2014), Enhanced homology-directed human genome engineering by controlled timing of CRSIPR/Cas9 delivery; eLife, 3 e04766 6) Li et al. (2023), Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair; Cell Biosci., 13 215
RT
TARGET: Cytoskeleton; Lysosome -- PATHWAY: Cell cycle; JNK pathway; Apoptosis inducer; Autophagy -- RESEARCH AREA: Cell death; Vesicles; CRISPR -- DISEASE AREA: Cancer