Inducer of definitive endoderm / IDE1 induces robust differentiation of embryonic stem cells into endoderm (EC50 = 125 nM) in both mouse and human cells via activation of the TGFß signaling pathway.1,2 It has been used in a simplified method to generate human microglia from pluripotent stem cells.3 IDE1 was also able to generate nephrogenic intermediate mesoderm cells from human pluripotent stem cells that had less toxicity and higher efficiently than cells generated with activin A.4 It was part of a step-wise all small molecule cocktail that was able to generate functional hepatic cells from human pluripotent stem cells.5
Biochemicals & reagents
1160927-48-9
2-[(E/Z)-(6-Carboxyhexanoylhydrazinylidene)methyl]benzoic acid
1) Borowiak et al. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells; Cell Stem Cell, 4 348 2) Hoveizi et al. (2014) Definitive endoderm differentiation of human-induced pluripotent stem cells using signaling molecules and IDE1 in three-dimensional polymer scaffold; J. Biomed. Mater. Res. A, 102 4027 3) McQuade et al. (2018) Development and validation of a simplified method to generate human microglia from pluripotent stem cells; Mol. Neurodegener. 13 67 4) Khoshdel-Rad et al. (2021) Promoting Maturation of Human Pluripotent Stem Cell-Derived Renal Microtissue by Incorporation of Endothelial and Mesenchymal Cells; Stem Cells Dev. 30 428 5) Pan et al. (2022) Efficiently generate functional hepatic cells from human pluripotent stem cells by complete small-molecule strategy; Stem Cell Res. Ther. 13 159
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PATHWAY: TGF-beta -- RESEARCH AREA: Stem cells