Ponasterone A
Inducer of ecdysone-inducible mammalian expression systems / Potent ecdysone agonist which elicits molting and metamorphosis in insects.1 Ponasterone A and related ecdysteroids have been used as activators in ecdysone-inducible mammalian expression systems.2,3 Has been used to create an inducible cell model for Huntingdon’s disease.4 Ecdysone analogs have been shown to display a strong antiapoptotic effect in mammalian cell lines thus ecdysone-based expression systems may not be suitable for analysis of apoptosis-related genes.5
Biochemicals & reagents
13408-56-5
25-Deoxy-2-hydroxyecdysone; 25-deoxyecdysterone
1. Gonsalves et al. (2011), Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster; BMC Genomics 12 475 2. Lafont and Dinan (2003), Practical uses for ecdysterones in mammals including humans: an update; J. Insect Sci. 3 7 3. Saez et al. (2000), Identification of ligands and coligands for the ecdysone-regulated gene switch; Proc Nat. Acad. Sci USA 97 14512 4. Chen et al. (2018), Heat shock promotes inclusion body formation of mutant huntingtin (mHtt) and alleviates mHtt-induced transcription factor dysfunction; J. Biol. Sci. 293 15581 5. Oehme et al. (2006), Agonists of an ecdysone-inducible mammalian expression system inhibit Fas Ligand- and TRAIL-induced apoptosis in the human colon carcinoma cell line RKO; Cell Death Differ. 13 189
-20°C
PATHWAY: Apoptosis -- RESEARCH AREA: Cell death