Trichostatin A
HDAC inhibitor / Trichostatin A (58880-19-6) is a potent and selective histone deacetylase (HDAC) inhibitor (Ki = 3.4 nM). Induces reversion of ras transformed cells to normal morphology. Trichostatin A induces dedifferentiation of primordial germ cells into embryonic germ cells. Cell permeable and active in vivo. Decreased global chromatin condensation increasing gene-editing efficiency 2-4X.4 Part of the CRISPY mix for increasing precise gene editing.5
Biochemicals & reagents
58880-19-6
TSA
1) Yoshida et al. (1990), Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A ; J. Biol. Chem., 265 17174 2) Futamura et al. (1995), Trichostatin A inhibits both ras-induced neurite outgrowth of PC12 cells and morphological transformation of NIH3T3 cells; Oncogene, 10 1119 3) Durcova-Hills et al. (2008), Reprogramming Primordial Germ Cells into Pluripotent Stem Cells; PLoS-One, 3 e3531 4) Molugu et al. (2023), Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells; CRISPR J., 6 473 5) Riesenberg and Maricic (2018), Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells; Nat. Commun., 9 2165
-20°C
TARGET: Protein deacetylase -- PATHWAY: Chromatin; Posttranslational modification; Cell cycle; Apoptosis inducer -- RESEARCH AREA: Epigenetics; Stem cells; Cell death -- DISEASE AREA: Infectious disease; Cancer