Bz-Val-Gly-Arg-AMC
Proteasome substrate / Bz-VGR-AMC may be used for measuring the trypsin-like activity of the 20S proteasome, in combination with Suc-LLVY-AMC, (for assaying its chymotrypsin-like activity) and Z-LLE-AMC, (for determining its PGPH activity). Fluorogenic substrate. Ex.: 380nm Em.: 460nm.
Biochemicals & reagents
87779-49-5
1) Gillardon et al. (2007), The 20s proteasome isolated from Alzheimer’s disease brainshows posr-translational modifications but unchanged proteolytic activity; J. Neurochem., 101 1483 2) Kim et al. (2002), Navigation inside a protease: substrate selection and product exit in tricorn protease from Thermoplasma acidophilum; J. Mol. Biol., 324 1041 3) Dahlmann et al. (2000), Different proteasome subtypes in a single tissue exhibit different enzymatic properties; J. Mol. Biol., 303 643 4) Lightcap et al. (2000), Proteasome inhibition measurements: clinical application; Clin. Chem., 46 673
-20°C
TARGET: Proteasome -- PATHWAY: Degradation -- RESEARCH AREA: Ubiquitin/Proteasome