Asunaprevir
HCV NS3 protease inhibitor/CRISPR enhancer / Asunaprevir is a potent hepatitis C virus NS3 protease inhibitor (IC50 = 0.2 – 3.5 nM).1 Asunaprevir was used as part of a repressible Cas9 system (small molecule-assisted shut off – SMASh) capable of degrading newly synthesized Cas9 protein rapidly to precisely control gene editing.2,3 The Asunapreivr/SMASh system has also been using to target endogenous PD-1 protein for proteasomal degradation.4
Biochemicals & reagents
630420-16-5
1) McPhee et al. (2012), Preclinical Profile and Characterization of the Hepatitis C Virus NS3 Protease Inhibitor Asunaprevir; Antimicrob. Agents Chemother. 56 5387 2) Chung et al. (2015), Tunable and reversible drug control of protein production via a self-excising degron; Nat. Chem. Biol. 11 713 3) Wu et al. (2020), A Small Molecule-Controlled Cas9 Repressible System; Mol.Ther. Nucleic Acids 19 922 4) Naruse et al. (2022), A degron system targeting endogenous PD-1 inhibits the growth of tumor cells in mice; NAR Cancer 4 zcac019
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TARGET: Protease -- RESEARCH AREA: CRISPR; Ubiquitin/Proteasome -- DISEASE AREA: Infectious disease