Phosphatidylethanol
PLD assay standard / Phospholipase D converts phosphatidylcholine to phosphatidylethanol in the presence of ethanol. This reaction is the basis of a sensitive and selective assay for PLD activity in intact cells for which this product may be used as a chromatographic standard.
Biochemicals & reagents
Unknown
1) Kobayashi et al. (1987), Phosphatidylethanol formation via transphosphatidylation by rat brain synaptosomal phospholipase D; J. Neurochem., 48 1597 2) Liscovith et al. (1989), Phosphatidylethanol biosynthesis in ethanol-exposed NG108-15 neuroblastoma X glimoa hybrid cells. Evidence for activation of a phospholipase D phosphatidyl transferase activity by protein kinase C; J. Biol. Chem., 264 1450 3) Pai et al. (1988), Phospholipase D catalyzes phospholipid metabolism in chemotactic peptide-stimulated HL-60 granulocytes; J. Biol. Chem., 263 12472
-20°C
TARGET: Phospholipase -- PATHWAY: Fatty acid metabolism