4-Bromo-A23187
Ca2+ ionophore (non-fluorescent) / Non-fluorescent analog1 of calcium ionophore A-231872 which may be used in experiments employing fluorescent probes to avoid interference3,4. Off-target effects include uncoupling of oxidative phosphorylation5 and inhibition of SR Ca2+-ATPase6.
Biochemicals & reagents
76455-48-6
4-Bromocalcimycin; 4-Bromo-calcium ionophore A2318
1 Deber et al. (1985) Bromo-A23187: A nonfluorescent calcium ionophore for use with fluorescent probes; Anal. Biochem. 146 349 2 Reed and Lardy (1972) A23187: a divalent cation ionophore; J. Biol. Chem. 247 6970 3 Wesseling et al. (2016) Phosphatidylserine Exposure in Human Red Blood Cells Depending on Cell Age; Cell Physiol. Biochem. 38 1376 4 Yang et al. (2013) NHERF2 protein mobility rate is determined by a unique C-terminal domain that is also necessary for its regulation of NHE3 protein in OK cells; J. Biol. Chem. 288 16960 5 Wong et al. (1973) Effects of antibiotic ionophore, A23187, on oxidative phosphorylation and calcium transport of liver mitochondria; Arch. Biochem. Biophys. 156 578 6 Hara and Kanazawa (1986) Selective inhibition by ionophore A23187 of the enzyme isomerization in the catalytic cycle of sarcoplasmic reticulum Ca2+-ATPase; J. Biol. Chem. 261 16584
-20°C
TARGET: Ionophore; ATPase -- PATHWAY: Calcium signaling; Mitochondrial function; Autophagy -- RESEARCH AREA: Cell death