SolisFAST® 1-step RT-PCR Kit with UNG Ready to Load

SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time. Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously. Combination of the highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results. The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons. Ready-to-Load variant comes with inert loading dyes included to directly load the samples on the gel to minimize steps and save time during analysis. Additionally, the kit is room temperature stable for up to 1 month due to the use of Stability TAG - a proprietary and patented polypeptide stabilization technology that is incorporated into each enzyme, enabling environmentally friendly ambient temperature shipping.

• Applications:Gene expression analysis; Determining the presence or absence of RNA; Virus detection; Cloning of RNA templates up to 2 kb; Suitable for both singleplex or multiplex (up to 5-targets) RT-PCR reactions