SolisFAST® 1-step RT-PCR Kit with UNG

SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time. Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously. Combination of the highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results. The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons. Additionally, the kit is room temperature stable for up to 1 month due to the use of Stability TAG - a proprietary and patented polypeptide stabilization technology that is incorporated into each enzyme, enabling environmentally friendly ambient temperature shipping.

• Applications:Gene expression analysis; Determining the presence or absence of RNA; Virus detection; Cloning of RNA templates up to 2 kb; Suitable for both singleplex or multiplex (up to 5-targets) RT-PCR reactions