HOT FIREPol® Multiplex qPCR Mix (Purple)

Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction. Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types. * Analyze 1-4 targets in 1 reaction; * High specificity and sensitivity; * Robust amplification of GC-rich targets; * Contains dUTP to prevent cross-contamination when used in combination with UNG. HOT FIREPol® Multiplex qPCR Mix (Purple) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and templates) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2, and Purple passive reference dye according to system requirements. HOT FIREPol® Multiplex qPCR Mix (Purple) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• Applications:Detection and quantification of DNA and cDNA targets; Profiling gene expression; Microbial detection; Viral load determination