Z-DEVD-FMK
Caspase3 inhibitor (cell permeable) / Z-DEVD-FMK (210344-95-9) is a cell-permeable trimethyl ester form of the irreversible caspase-3 inhibitor DEVD-FMK (fluoromethylketone) with IC50 = 130 nM.1,2 Methyl esters confer cell permeability and are hydrolyzed to the active COOH form via intracellular esterases. Inhibits caspase-3-dependent apoptosis and pyroptosis in a variety of cell lines.3,4 Active in vivo.5
Biochemicals & reagents
210344-95-9
1 Nicholson et al. (1995), Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis; Nature, 376 37 2 Brockstedt et al. (1998), Identification of apoptosis-associated proteins in a human Burkitt lymphoma cell line. Cleavage of heterogenous nuclear ribonucleoprotein A1 by caspase 3; J. Biol. Chem., 273 28057 3 Wang et al. (2022), Osthole Induces Apoptosis and Caspase-3/GSDME-Dependent Pyroptosis via NQO1-Mediated ROS Generation in HeLa Cells; Oxid. Med. Cell. Longev., 2022 8585598 4 Jung et al. (2022), Withanolide modulates the potential crosstalk between apoptosis and autophagy in different colorectal cancer cell lines; Eur. J. Pharmacol., 928 175113 5 Knoblach et al. (2004), Caspase inhibitor Z-DEVD-FMK attenuates calpain and necrotic cell death in vitro and after traumatic brain injury; J. Cereb. Blood Flow Metab., 24 1119
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TARGET: Protease -- PATHWAY: Apoptosis inhibitor; Pyroptosis -- RESEARCH AREA: Cell death; Neuroscience -- DISEASE AREA: Neurodegeneration