Filipin III
Cholesterol probe / Filipin III is a polyene macrolide antibiotic. It may be used in a double staining procedure for the detection of lipoproteins1. Binds specifically to cholesterol forming a fluorescent complex which may be used for staining2. Disrupts the lipid raft/caveolae endocytosis pathway3. Prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release4.
Biochemicals & reagents
480-49-9
1) Smejkal and Hof et al. (1994), cholesterol-specific probe for lipoproteins immobilized on nitrocellulose membranes; Biotechniques, 16 68 2) Bornig and Geyer et al. (1974), Staining of cholesterol with the fluorescent antibiotic “filipin”; Acta Histochemica, 50 110 3) Huo et al. (2003), Lipid rafts/caveolae are essential for insulin-like growth factor-1 receptor signaling during 3T3-L1 preadipocyte differentiation induction; J. Biol. Chem., 278 11561 4) Marella et al. (2002), Filipin prevents pathological prion protein accumulation by reducing endocytosis and inducing cellular PrP release; J. Biol. Chem., 277 25457
-20°C / Protect from light
TARGET: Substrate / Probe; Prions -- PATHWAY: Intracellular transport; Cholesterol metabolism -- DISEASE AREA: Atherosclerosis