PITSTOP2
Clathrin-mediated endocystosis inhibitor / PITSTOP2 inhibits clathrin-mediated endocytosis via blockade of endocytic ligand association with the clathrin terminal domain leading to clathrin coat assembly inhibition.1 It was able to cause a collapse of the nucleocytoplasmic permeability barrier and a reduction of Importin-ß binding.2 PITSTOP2 reduced viability in HeLa cells by trapping dividing cells in metaphase through loss of mitotic spindle integrity.3
Biochemicals & reagents
1332879-52-3
1) Von Kleist et al. (2011) Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition; Cell 146 P471 2) Liashkovich et al. (2015) Clathrin inhibitor Pitstop-2 disrupts the nuclear pore complex permeability barrier; Sci. Reports 5 9994 3) Smith et al. (2013) Inhibition of clathrin by pitstop2 activated the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells; Mol. Cancer 12 4
-20°C
TARGET: Cytoskeleton; GTPase -- PATHWAY: Intracellular transport; Cell migration; ERK -- RESEARCH AREA: Vesicles -- DISEASE AREA: Cancer; Infectious diseasePain