HOT FIREPol® EvaGreen® HRM Mix (ROX)

Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis. * Highly sensitive mix for accurate SNP detection; * Sensitive EvaGreen® dye for reliable HRM performance; * Reaction set-up without dry ice. High-Resolution Melt (HRM) analysis is a fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations, and DNA sequence variations. HOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for HRM analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye, and ROX dye according to system requirements. The user simply needs to add water, template, and primers. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers.

• Applications:High-Resolution Melt (HRM); SNP detection; dye-based qPCR