HOT FIREPol® Probe qPCR Mix Plus (ROX)

Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry. Cost-effective real-time qPCR master mix for probe-based qPCR assays. This master mix has been developed for TaqMan® probes but is suitable for other hydrolysis probe types as well. * Suitable for singleplex and duplex assays; * High specificity and sensitivity; * Reaction set-up and shipment without dry ice. HOT FIREPol® Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• Applications:Detection and quantification of DNA and cDNA targets; Profiling gene expression; Microbial detection; Viral load determination