EAE kit

Turnkey solution for multiple sclerosis (MS) studies. Each kit provided allows the induction of EAE in 10 mice. The kit contains 2.5mL of MOG35-55/CFA emulsion, one vial containing 5µg pertussis toxin (PTX) in glycerol buffer and a recommended experimental protocol with typical results. MOG35-55/CFA emulsion: Myelin Oligodendrocyte Glucoprotein (MOG) is used in emulsion with Complete Freund’s Adjuvant (CFA) in EAE studies. Emulsification is an important step in the success of the study, results will depend on this phase. MOG/CFA emulsion is provided pre-filled in ready-to-use syringes. The complete Freund’s adjuvant is used as an immunopotentiator. It is composed of inactivated and dried mycobacteria and used with MOG to induce EAE. Experimental autoimmune encephalomyelitis is the model commonly used to study efficacy of drugs for treatment of multiple sclerosis (MS) but also pathogenesis of autoimmunity, CNS inflammation, demyelination, cell trafficking and tolerance induction. Why pre-filled MOG/CFA emulsion? Two emulsion preparations that are not exactly equivalent can give very different outcomes. Indeed several critical steps are important, such as quantities used, emulsification time, etc. Pre-filled MOG/CFA emulsion is an ideal solution for your experiments, it provides reliable, reproducible and repeatable results. Moreover, it saves you precious time when performing your tests. MOG1-125: Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in central nervous system (CNS) on the outermost surface of myelin sheaths. MOG protein is a crucial autoantigen for multiple sclerosis. MOG1-125 corresponds to the extracellular domain of MOG protein along with a 6x His tag is expressed in E. Coli. Numerous studies have shown that MOG1-125 specifically induces B-cell immunity. For your therapeutic models targeting B cells, please contact us.