- In this section:
- Exo-spin™ Purification Kits (EX01 for cell culture media, urine & saliva)
- Exo-spin™ Purification Kits (EX02 for blood sera and plasma)
- Exo-spin™ Columns (EX03 for small concentrated samples)
- Exo-spin™ midi Columns (EX04 for larger concentrated samples)
- Exo-spin™ mini-HD Columns (EX05 for intermediate concentrated samples)
- Exo-spin™ 96 (EX07 for blood sera and plasma)
The Exo-spin™ kits generate superior yields and higher quality whole exosomes than traditional methods, with no need for ultracentrifugation or organic buffers. Exo-spin purifies particles in the range 30 – 400nm, the same size range achieved with ultracentrifugation. Exo-spin technology is superior to techniques which rely solely on precipitation where co-purification of large amounts of non-exosomal proteins and other material can occur as well as carry over of the precipitant.
Exo-spin kits and columns are designed to isolate pure, functional exosomes from a variety of biological fluids including cell culture media, urine and saliva, blood plasma and sera. Exo-spin purified exosomes are suitable for a variety of downstream applications including RNA analysis, protein analysis, mass spec and functional use.
Exo-spin is offered in several configurations and options for use. Kits for large volume, low exosome-containing samples employ a precipitation step followed by size exclusion column chromatography. For samples with high exosome concentrations like sera and plasma or for mass spec applications, the precipitation step can be omitted. Refer to the following product selection guide to identify the correct product for your applications and sample type.
Key Features |
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Excellent yields and high levels of purity |
No ultracentrifugation |
Simple and reliable |
Exosomes with low levels of protein and rRNA contamination |
The protocol provides consistent results every time |
Isolate intact whole exosomes for functional studies |
Low exosome-content biofluids
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Sample Volume |
isolation method |
Exo-spin Kit |
column bed length |
< 1ml to 50 ml |
concentration + SEC |
EX01 mini |
1.3 cm |
< 1 ml to 75 ml Note 1 |
concentration + SEC |
EX05 mini-HD |
6.35 cm |
75 ml to 500 ml Note 1 | concentration + SEC | EX04 midi | 2.15 cm |
Plasma and Sera |
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Sample Volume |
isolation method |
Exo-spin Kit |
column bed length |
< 100 µl Note 2 |
SEC |
EX03 mini columns |
1.3 cm |
< 150 µl |
SEC |
EX05 mini-HD | 6.35 cm |
100 µl - 250 µl Note 3 | concentration + SEC | EX02 mini blood | 1.3 cm |
1 ml | SEC | EX04 midi | 2.15 cm |
Publications
In this section
- Exo-spin™ Purification Kits (EX01 for cell culture media, urine & saliva)
- Exo-spin™ Purification Kits (EX02 for blood sera and plasma)
- Exo-spin™ Columns (EX03 for small concentrated samples)
- Exo-spin™ midi Columns (EX04 for larger concentrated samples)
- Exo-spin™ mini-HD Columns (EX05 for intermediate concentrated samples)
- Exo-spin™ 96 (EX07 for blood sera and plasma)