Exo-spin™ midi Columns (EX04 for larger concentrated samples)

For the direct purification of sample sizes up to 1 ml. Larger sample volumes can be used with prior precipitation.

 

 

Exo-spin™ midi columns are a versatile, quick and effective way to isolate and purify exosomes directly without precipitation from a range of samples like blood sera and cell culture medium. No precipitation is required for volumes up to 1ml making these columns an ideal choice for applications like mass spec which is not compatible with the use of precipitants. Cell debris, proteins and other impurities are effectively removed yielding a clean exosome sample in less than 30 minutes.

 

Brook AC, Jenkins RH, Clayton A, Kift-Morgan A, Baby AC, Shephard AP, Mariotti B, Cuff SM, Bazzoni F, Bowen T, Fraser DJ, and Eberl M. (2019). Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis. Scientific Reports 9(1):10136. 

Salimu J, Webber J, Gurney M, Al-Taei S, Clayton A and Tabi Z. (2017). Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes. Journal of Extracellular Vesicles 6(1):1368823.

Santangelo L, Giurato G, Cicchini C, Montaldo C, Mancone C, Tarallo R, Battistelli C, Alonzi T, Weisz A and Tripodi M. (2016). The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting. Cell Reports 17(3):799-808.

Welton JL, Webber JP, Botos LA, Jones M and Clayton A. (2015). Ready-made chromatography columns for extracellular vesicle isolation from plasma. Journal of Extracellular Vesicles 4:27269