FFPE ChIP

A solid phase chromatin immunoprecipitation assay from chromatin extracted from Formalin Fixed Paraffin Embedded (FFPE) tissue. The Chromatrap® FFPE ChIP kit has overcome the issues often associated with FFPE ChIP, by offering a streamlined protocol which provides the user with good quality isolated chromatin.

Ideal for tissue samples, biopsies and difficult to lyse samples. The sensitivity of the unique solid state platform ensures that small samples can be used with efficient enrichment of both high and low abundant targets. Chromatrap® FFPE ChIP kits provide a quicker, easier and more efficient way of performing ChIP assays from a range of FFPE samples. Using the unique, patented Chromatrap® technology maximizes the capture efficiency of the target chromatin/antibody complex. By avoiding the use of magnetic or agarose beads, non-specific background is eliminated together with reduced manual handling errors and subsequent sample loss.

• This kit is compatible with both qPCR and sequencing as a downstream process and has a wide dynamic range, ensuring that adequate DNA is enriched for library preparation for sequencing. The wide dynamic range allows for better results from small sample sizes and provides greater flexibility
• Prior to sequencing, DNA purification is required and Chromatrap® purification columns are supplied for ease of use.
• The kits contain sufficient Protein A or Protein G based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.

Chromatin was extracted from rat FFPE uterine tissue and immunoprecipitated for the high abundant mark H3 using Chromatrap Protein A FFPE ChIP Kit and compared against a competitor kit. The graph shows level of enrichment of H3 onto the GAPDH locus to be 4x that of the leading competitor, with excellent signal to noise ratio.