Coronavirus
The hybrid alpha-pseudovirus for SARS-CoV-2 (Ha-CoV-2) is a newly developed SARS-CoV-2 virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid report expression (luciferase, GFP or RFP) in target cells. Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, Ha-CoV-2 is assembled with all 4 structural proteins (S, M, N, and E) of SARS-CoV-2, and contains a reporter genome derived from an alphavirus-based vector for rapid (6 hours) and robust expression of reporter genes. The alphavirus vector does not contain any of the structural proteins from the authentic virus and the alpha-pseudovirus particles are single-cycle.
Applications
- Screening and quantification of anti-coronavirus neutralizing antibodies
- Anti-coronavirus drug screening
- Quantification of viral mutant infectivity
- Identification of host co-factors and restriction factors of coronaviruses
SARS-CoV-2 Lineage
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* (Share the Same Spike Mutations)