Cysteine protease inhibitor / Cell-permeable derivative of E-64c, an inhibitor of calpain and other cysteine proteases, such as papain, cathepsin B and cathepsin L.1,2 Addition to cell cultures should be done in serum-free media as esterases in serum will cleave the ethyl ester and reduce cell permeability. Typical working concentration is 0.5-10 µg/ml. Inhibits degradation of autophagic cargo inside autolysosomes3
Biochemicals & reagents
88321-09-9
Aloxistatin; Loxistatin
1) McGowan et al. (1989), Inhibition of calpain in intact platelets by the thiol protease inhibitor E-64d; Biochem. Biophys. Res. Commun., 158 432 2) Wilcox and Mason (1992), Inhibition of cysteine proteinases in lysosomes and whole cells; Biochem. J., 285 495 3) Mizushima et al. (2010), Methods in mammalian autophagy research; Cell, 140 313
-20°C
TARGET: Protease -- PATHWAY: Autophagy; Degradation -- RESEARCH AREA: Cell death; Vesicles