IL-35 Heterodimer, human recombinant
Interleukin-35 (IL-35) is a member of IL-12 cytokine family and is produced by regulatory T-cells (Tregs). This heterodimeric cytokine is comprised of one p35 subunit (also a subunit of IL-12) and one EBI3 subunit (also a subunit of IL-27). IL-35 signals through IL-12Rbeta2 and gp130 heterodimers and homodimers to induce a suppression of inflammatory responses. Recombinant human IL-35 is a single chain product, containing 442 amino acids, with a predicted molecular weight of 49 kDa (observed molecular weight of approximately 75-95 KDa reduced and approximately 65-75 kDa unreduced). From N terminus to C terminus, the molecule is comprised of a poly His tag, the EBI3 subunit, a G rich linker and the p35 subunit.
Peptides & proteins
-20°C
NAME: Interleukin-35; ACCESSION/UNIPROT#: Q14213/P29459; EXPRESSION SYSTEM: HEK 293 cells; FORMAT: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 30 mM sodium chloride, pH7.5. Reconstitute in sterile water at 0.1 mg/mL.; #AA: 406; SEQUENCE: EBI3:RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK p35:RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS