PODS® Human TGF-β 3

PODS® Human TGF-β3 crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® crystals generates the same peak dose as 3.3 µg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® crystals that is optimal for a particular experiment should be determined empirically, using 50 million PODS® crystals equivalence to 3.3 µg of standard growth factor is a good starting point. To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.