HOT FIREPol® EvaGreen® qPCR Supermix

Optimized ready-to-use solution for dye-based real-time quantitative PCR assays. This master mix has been developed to give highly specific and sensitive results and demonstrates excellent performance with GC-rich regions. * High sensitivity with low DNA concentrations; * Blue dye to ease pipetting; * Reduced primer dimer formation. One qPCR mix for all cyclers (except capillary and high ROX* cyclers); * Contains dUTP to prevent carryover; * Contamination with UNG treatment. HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments. The Mix contains HOT FIREPol® DNA Polymerase and a passive internal reference dye that is compatible with most qPCR cyclers including no ROX and low ROX* reference signal requiring platforms. The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.

• Applications:Detection and quantification of DNA and cDNA targets; Profiling gene expression; Microbial detection; Viral load determination