HOT FIREPol® Blend Master Mix with 12.5 mM MgCl2

Hot-start PCR master mix for more demanding assays. *Increased yield, sensitivity and specificity; *Up to 5x higher fidelity; *Suitable for templates up to 5 kb; *Reduced primer dimer formation. HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water). HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase. In addition, the mix also allows the amplification of longer fragments. Generated PCR products are compatible with blunt-end and T/A cloning procedures.

• Applications:Endpoint PCR; Genotyping; Colony PCR; TA cloning