SOLIScript® 1-step Multiplex Probe Kit (ROX) (250 rxn)

Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water. * cDNA synthesis up to 60°C for superior specificity; * Analyze 1-4 targets in 1 reaction; * Reaction set-up and shipment without dry ice; * Robust amplification of GC-rich targets; * Contains dUTP to prevent cross-contamination when used in combination with UNG; * RNase inhibitor included in kit; * Includes ROX reference dye. SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

• Applications:Detection and quantification of RNA targets with qPCR; DNA/LNA hydrolysis probe-based assays; Suitable for singleplex and multiplex reactions