SOLIScript® KIT (200 rxn)

Reverse transcriptase with high thermostability and superior specificity. Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature. * Superior performance with gene-specific primers; * Reaction temperatures from 37°C to 60°C; * Reaction set-up and shipment at room temperature; * NB! not compatible with random primers. SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase. SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C). SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity.

• Applications:First-strand cDNA synthesis; RT-PCR; RT-qPCR