SoliSD™ Bsm DNA Polymerase Kit
SoliSD™ Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C. The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loop-mediated isothermal amplification (LAMP).
- Applications:Isothermal amplification
Molecular Biology
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).
-20°C until Expiry Date; 4°C for up to 6 months; room temperature for at least 1 month; Freeze-thaw stability: 10 cycles
Unit definition: The unit is determined in DNA strand displacement assay, where 1 unit of the enzyme catalyzes displacement of 40 nmol of oligonucleotide probe from the complementary DNA strand in 30 min at 60 °C.