|
Introducing a revolution in growth factor quality, stability and bioavailability - PODS® Growth Factors. From Activin to VEGF - more than 70 different growth factors are now available in this novel format. PODS Growth Factors are slow-release microcrystals made by the co-expression of growth factor with an inert polyhedrin scaffold protein. Each PODS microcrystal contains millions of copies of the cargo protein which are released over time by endogenous proteases secreted by cells or present in sera. |
|
Key Features/Benefits
- Highly stable storage in liquid at 4°C to 25°C
- Made in insect cells for important post-translational modifications
- Slow release mechanism
- Reduce frequency of media changes
- Steadier growth factor concentrations over time
- 100% bioactivity and product consistency for more reproducible results
- Easily create localized deposits to produce physiologically relevant concentration gradients
PODS® Applications Include:
- Micropatterning
- Physiological, stable gradient formation
- Bioinks for 3D printing
- Microcarriers
- Functionalizing scaffolds
- Microfluidics (lab on a chip)
- Improved and simplified stem cell culture
- Therapeutic protein delivery
Usage Recommendations
The concentration to which a growth factor accumulates in cell culture media (or in-vivo environment) will depend on the amount of PODS added, the rate of cargo release, and the subsequent rate of degradation of the released cargo protein. As a rule of thumb, in the presence of 10% serum, peak levels of available growth factors released from PODS are reached within 24-48 hours. Typically, at this point 20% of the growth factor cargo initially contained within the PODS is present in a soluble form and available to cells. For example, if PODS containing 100 ng of cargo are added to 10 ml of cell culture media containing 10% serum, it can be expected that 20 ng will be released after 24 hours to give a concentration of available growth factor of 2 ng/ml. The concentration that you need for a particular application will likely be lower than the equivalent conventional growth factor. This is because PODS are better at maintaining minimum growth factor concentrations. Pre-incubating PODS with serum for 24 hours prior to culture will ensure that available growth factor is immediately present. Ultimately, the amount of PODS growth factor that is optimal for a particular experiment should be optimized empirically.
Additional Information
PODS™ Human Growth Factors
|
|
PODS™ Mouse Growth Factors
PODS™ Rat Growth Factors
Additional PODS™
|
Empty (PODS without cargo protein for validation experiments) |