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  2. qPCR Mix Compatibility Table: Probe-based qPCR Mixes

qPCR Mix Compatibility Table: Probe-based qPCR Mixes

Agilent/Stratagene

Mx3000P™, Mx3005P™, Mx4000™

Fast cycling
  • SolisFAST® Probe qPCR Mix (ROX)*
  • SolisFAST® Probe qPCR Mix with UNG (ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix (ROX)*
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (ROX)*
Applied Biosystems

5700, 7000, 7300, 7700, 7900HT, 7900HT Fast, StepOne™, StepOnePlus™

Fast cycling
  • SolisFAST® Probe qPCR Mix (ROX)*
  • SolisFAST® Probe qPCR Mix with UNG (ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix (ROX)*
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (ROX)*

 

7500, 7500 Fast, ViiA™7, QuantStudio™ 3**, 5, 6 Flex, 7 Flex, 12K Flex

Fast cycling
  • SolisFAST® Probe qPCR Mix (ROX)*
  • SolisFAST® Probe qPCR Mix with UNG (ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix (ROX)*
  • HOT FIREPol® Multiplex qPCR Mix (Purple)**
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (ROX)*

** Mixes with Purple reference dye are not compatible with Applied Biosystems QuantStudio™ 3

Bio Molecular Systems (BMS)

Mic

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Bio-Rad

CFX96™, CFX384™, iQ™5, MyiQ™, Chromo4™, Opticon®2; MiniOpticon®

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Eppendorf

Mastercycler® ep Realplex

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Illumina

The Eco™

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Qiagen

Rotor-Gene® 3000, Rotor-Gene® 6000, Rotor-Gene® Q

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Roche Applied Science

LightCycler® 480, LightCycler® Nano, LightCycler® 96

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)

 

LightCycler® 1.x, 2.0

Standard cycling
  • HOT FIREPol® Probe qPCR Mix Plus (Capillary)
Takara

Thermal Cycler Dice™ (TP800)

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Thermo Scientific

PikoReal™

Fast cycling
  • SolisFAST® Probe qPCR Mix (no ROX)
  • SolisFAST® Probe qPCR Mix with UNG (no ROX)
Standard cycling
  • HOT FIREPol® Multiplex qPCR Mix
  • HOT FIREPol® Probe Universal qPCR Mix*
  • HOT FIREPol® Probe qPCR Mix Plus (no ROX)

* Mixes that contain ROX can not be used with ROX, JUN and Texas Red labelled probes.