TRIFic™ Exosome Assay Kits

TRIFic™ exosome assays quickly provide quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma. TRIFic exosome assays are being developed for widely used markers of exosomes; the tetraspanin proteins CD9, CD63 and CD81.  Kits come complete including control exosomes for standard curves. TRIFic exosome assays deliver clear, consistent data for the following reasons:

• Simplicity of the assay results in a high degree of reproducibility.
• Europium is used for detection, providing a high degrees of sensitivity.
• Only antigens held in association in multiple copies (such as tetraspanins in exosomes) are detectable with the assay, resulting in specificity.

TRIFic Exosome Assay Schematic

Step 1: Biotinylated antibody is bound to streptavidin coated assay plates.

Step 2: Biological samples are added. Exosomes and any free antigen are captured by the antibody.

Step 3: Europium labeled antibody is added and binds specically to exosome antigen. Samples are read in a time resolved fluorescence plate reader.

Readings obtained using the TRIFic exosome assay with the provided standard (exosomes purified from LNCaP cells). Note the linearity of response over a wide range of concentrations.