AimPlex™ multiplex assays utilize bead populations differentiated by size and fluorescence intensity levels allowing for measurements up to 24 analytes simultaneously in a single reaction.  AimPlex assays are read on a flow cytometer equipped with a 488nm laser with the maximum emission of the bead classification dye at 700 nm.

 

Similar in principle to a sandwich ELISA, each bead population conjugated with a specific capture antibody traps the protein of interest in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein followed by a streptavidin-R-phycoerythrin treatment. Sample protein concentrations are determined by comparing the fluorescent signals to a standard curve generated from serial dilutions of a known analyte concentrations.