Fast and robust reverse transcriptase for wide range of applications. Genetically modified MMLV-based reverse transcriptase with increased thermostability and improved performance at elevated temperatures. * High specificity and yield; * Wide reaction temperature from 37°C to 60°C; * Fast 15 min reaction time; * A simple 2-vial kit with enzyme and reaction buffer only; * Reaction set-up and shipment without ice. FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT-qPCR (quantitative reverse transcription PCR).
First-strand cDNA synthesis;
RT-PCR;
RT-qPCR
