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T4 Enzymes

T4 DNA Ligase
  • Cloning of restriction enzyme generated DNA fragments
  • Cloning of PCR products
  • Next-gen library preparation
  • Joining linkers and adapters to cohesive or blunt-ended DNA
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Self-circularization of linear DNA
T4 DNA Polymerase
  • 3´-overhang removal to form blunt ends
  • 5´-overhang fill-in to form blunt ends
  • Probe labeling using replacement synthesis
  • DNA library preparation for next-generation sequencing
  • Ligation-independent cloning of PCR products
  • Second strand synthesis in site-directed mutagenesis
T4 Polynucleotide Kinase (PNK)
  • Labeling of 5'-termini of nucleic acids
  • 5'-phosphorylation of oligonucleotides, PCR products, and other DNA or RNA prior to ligation
  • Phosphorylation of PCR primers
  • Detection of DNA modification by the [32P]-postlabeling assay
  • Removal of 3'-phosphate groups

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