life science research products, biological research products, biotechnology
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Spin Trapping

Spin traps react with free radicals in solution to yield stable adducts which can be observed by EPR spectroscopy. The nitrone spin trap DMPO is widely used to detect free radicals, and is particularly useful for identifying oxygen-centered radicals like superoxide and hydroxyl radicals as well as C, N and S-centered radicals due to the characteristic EPR spectra of the adducts. BMPO is another useful spin trap to detect and identify the structure of reactive oxygen-centered radicals with a significantly longer half-life of its superoxide radical adduct. Both spin traps (DMPO and BMPO) are cell permeable making them useful for detecting extracellular and intracellular reactive oxygen species in vivo.
 

DMPO 

Application: EPR (ESR) detection, immuno-spin trapping method
  • Ultra high purity
  • No pre-purification required
DMPO is provided at high purity and does not require any pre-purification as compared to most commercially available DMPO which require further purification to remove impurities that cause high backgrounds.
 
DMPO
 
Figure 1: ESR spectra were taken in the presence of hydroxy radicals generated by Fenton reaction (black) and blank (blue). Cat# D048-10 gives very clear signal and higher S/N ratio compared to DMPO from two common suppliers.
 
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BMPO

Application: EPR (ESR) detection, superoxide anion radical
  • Longer half-life versus other reagents
  • High solubility in water
  • Greater S/N ratio
BMPO detects both superoxide and hydroxyl radicals and shows a much longer half-life (t1/2=24 min) than other spin trap reagents.
 
BMPO
 
Figure 2: Half-life of BMPO. BMPO is applicable for experiments which require longer handling times (ex. enzyme reactions)
 
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