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Exosome Tracking

ExoFLARE™ Exosome Tracking Kits

ExoFLARE kits include transfection-ready plasmids to transiently or stably express human tetraspanin proteins tagged with a unique protein tag (FLARE - FLuorescence Activating Response Element) in your cells of interest. FLARE-tagged proteins are detected with a tag-specific fluorophore to allow tracking before and after incorporation into exosomes for extensive periods.

Neither the FLARE tag or dye fluoresce alone. When the FLARE protein and dye combine, a conformational change in the dye structure occurs resulting in fluorescence. There is continuous turnover of the dye for sustained fluorescence, but without the levels of photo-bleaching associated with fluorescent proteins, making the system ideal for long-term tracking. ExoFLARE dyes are offered in both cell permeable and cell impermeable formats offering flexibility in experimental design.

exosometracking

For long-term tracking of exosomes, use ExoFLARE™ to:

  • Image individual exosomes
  • Track long term movement of exosomes
  • Detect uptake of exosomes in non-transfected recipient cells

Key Attributes

  • Significantly brighter than fluorescent proteins
  • Differentiate between donor and recipient cell exosomes
  • Low background levels
  • Non-toxic
exosometracking

Figure 1: ExoFLARE constructs were transiently transfected into DU154N cells. ExoFLARE cell-permeable dye was added to the media and cells were imaged using a confocal fluorescence microscope. Red = ExoFLARE; Green = Hoechst.

Kit Contents

  • 20 µg of a mammalian expression construct (for a single CD antigen)
  • EF Red s dye
  • Buffers
  • User Guide

ExoFLARE Kits

Construct

CD9

CD63

CD81

With Cell Permeable Dye

EX304

EX305

EX306

With Cell Impermeable Dye

EX301

EX302

EX303

Additional Fluorophore

Type

Cell permeable

Cell impermeable

100 and 1000 reactions

EX402-100, EX402-1000

EX401-100, EX401-1000