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Exosome Detection

TRIFic™ Exosome Assays

rapid exosome detection and quantitation from any sample

TRIFic™ exosome assays quickly provide quantitative data from purified or unpurified samples, including direct measurement of exosomes in plasma. TRIFic exosome assays are being developed for widely used markers of exosomes; the tetraspanin proteins CD9, CD63 and CD81. Kits come complete including control exosomes for standard curves. TRIFic exosome assays deliver clear, consistent data for the following reasons:

  • Simplicity of the assay results in a high degree of reproducibility.
  • Europium is used for detection, providing a high degrees of sensitivity.
  • Only antigens held in association in multiple copies (such as tetraspanins in exosomes) are detectable with the assay, resulting in specificity.

TRIFic Exosome Assay Schematic

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Step 1: Biotinylated antibody is bound to streptavidin coated assay plates.

Step 2: Biological samples are added. Exosomes and any free antigen are captured by the antibody.

Step 3: Europium labeled antibody is added and binds specically to exosome antigen. Samples are read in a time resolved fluorescence plate reader.

standard

Readings obtained using the TRIFic exosome assay with the provided standard (exosomes purified from LNCaP cells). Note the linearity of response over a wide range of concentrations.

Products

Note: product availability depends on country. Product detail pages show availability of each product.

TRIFic™ Exosome Assay, human CD9

TRIFic™ Exosome Assay, human CD63

TRIFic™ Exosome Assay, human CD81